منابع مشابه
Colchicine Binding in Tissue Slices
INTRODUCTION vitro . Under appropriate conditions changes in colchicine binding should reflect changes in the microtubule-subunit equilibrium . The effects of both cAMP and calcium on colchicine binding were examined. Calcium was included in this study, for two reasons . First, it is necessary for some of the functions that have been linked with microtubules, and second, cAMP is known to alter ...
متن کاملCOLCHICINE BINDING IN TISSUE SLICES Decrease by Calcium and Biphasic Effect of Adenosine-3', 5'-Monophosphate
INTRODUCTION vitro . Under appropriate conditions changes in colchicine binding should reflect changes in the microtubule-subunit equilibrium . The effects of both cAMP and calcium on colchicine binding were examined. Calcium was included in this study, for two reasons . First, it is necessary for some of the functions that have been linked with microtubules, and second, cAMP is known to alter ...
متن کاملEffect of Cytochalasin B and Colchicine on Α-amylase Release from Rat Parotid Tissue Slices
We have recently reported (3) that cytochalasin B, which disrupts microfilaments, and colchicine, which disrupts microtubules, interfered with the release of a-amylase from rat parotid tissue slices . Our observations on the effect of cytochalasin B on a-amylase release have been confirmed by Rifkin et al . (14) . Inhibitory actions of cytochalasin B or colchicine in several other systems have ...
متن کاملBinding of Colchicine-3h to Vinblastine- and Vincristine-induced Crystals in Mammalian Tissue Culture Cells
the vinca alkaloids, vinblastine sulfate and vincristine sulfate (Velban and Oncovin, respectively ; Eli Lilly and Co ., Indianapolis, Ind .), cause hypertrophy of the granular endoplasmic reticulum and annulate lamellae in cultured mammalian cells (7), and the accumulation of large proteinaceous cytoplasmic crystals in tissue culture cells (8), in cells of the central nervous system (9), and i...
متن کاملSegmentation of Axonal Fibres in Tissue Slices
This work focuses on the segmentation of axonal structures in digital images of organotypic slice co-cultures. An image processing chain is presented, which relies on anisotropic diffusion for preprocessing of the images and the intelligent scissors method for segmentation. This method requires manual user interaction to set the starting points. To overcome this drawback, the initial parameters...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1971
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.50.2.544